cosmc antibody Search Results


mouse anti human cosmc primary antibody  (Santa Cruz Biotechnology)
93
Santa Cruz Biotechnology mouse anti human cosmc primary antibody
Mouse Anti Human Cosmc Primary Antibody, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cosmc+antibody/Cosmc+Antibody/10__2147_slash_cmar__s214553-45-8-14
Average 93 stars, based on 1 article reviews
mouse anti human cosmc primary antibody - by Bioz Stars, 2026-11
93/100 stars
  Buy from Supplier
cosmc antibody  (Proteintech)
93
Proteintech cosmc antibody
Expression of aberrant O-glycans in pancreatic cancer. a Biosynthesis of Tn <t>antigen,</t> <t>sTn</t> antigen and Core1 and 3 structures. Tn antigen is composed of an O-glycosidic linked N -acetylgalactosamine (GalNAc) to the –OH group of serine/threonine (S/T). Tn antigen is either processed by core 1 T-synthase (C1GalT1) and its chaperone <t>(COSMC),</t> which transfers a galactose (Gal) to GalNAc-serine/threonine to form the T antigen also referred as core 1 structure or processed by transfer of a N -acetylglucosamine (GlcNAc) to form the core 3 structure. Tn antigen can also be modified by addition of a sialic acid (NeuAc). b Differential expression of Tn antigen in pancreatic carcinoma cell lines. Eight different PDAC cell lines were available for analysis. Western and Far-Western blot analysis of total cell lysates was performed using the Tn antigen specific antibody MA1-80055. Detection of HSC70 served as loading control. Jurkat cells were used as positive control for Tn antigen expression. c Expression of Tn antigen and aberrant O-glycans in COSMC knockdown cells. Western blot analysis showed a strong expression of aberrant O-glycans as well as Tn antigen in Panc-1 COSMC knockdown cells compared to control cells. Sialyl-Tn and Tn antibodies were used as well as lectins such as VVL ( Vicia villosa lectin) and WFL ( Wisteria floribunda lectin)
Cosmc Antibody, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cosmc+antibody/COSMC+Antibody/pmc04447007-235-17-20
Average 93 stars, based on 1 article reviews
cosmc antibody - by Bioz Stars, 2026-11
93/100 stars
  Buy from Supplier
β3 polyclonal antibody  (Boster Bio)
90
Boster Bio β3 polyclonal antibody
Expression of aberrant O-glycans in pancreatic cancer. a Biosynthesis of Tn <t>antigen,</t> <t>sTn</t> antigen and Core1 and 3 structures. Tn antigen is composed of an O-glycosidic linked N -acetylgalactosamine (GalNAc) to the –OH group of serine/threonine (S/T). Tn antigen is either processed by core 1 T-synthase (C1GalT1) and its chaperone <t>(COSMC),</t> which transfers a galactose (Gal) to GalNAc-serine/threonine to form the T antigen also referred as core 1 structure or processed by transfer of a N -acetylglucosamine (GlcNAc) to form the core 3 structure. Tn antigen can also be modified by addition of a sialic acid (NeuAc). b Differential expression of Tn antigen in pancreatic carcinoma cell lines. Eight different PDAC cell lines were available for analysis. Western and Far-Western blot analysis of total cell lysates was performed using the Tn antigen specific antibody MA1-80055. Detection of HSC70 served as loading control. Jurkat cells were used as positive control for Tn antigen expression. c Expression of Tn antigen and aberrant O-glycans in COSMC knockdown cells. Western blot analysis showed a strong expression of aberrant O-glycans as well as Tn antigen in Panc-1 COSMC knockdown cells compared to control cells. Sialyl-Tn and Tn antibodies were used as well as lectins such as VVL ( Vicia villosa lectin) and WFL ( Wisteria floribunda lectin)
β3 Polyclonal Antibody, supplied by Boster Bio, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cosmc+antibody/Anti-C1GALT1-specific+chaperone+1+C1GALT1C1+Antibody/pmc03131568-155-4-10
Average 90 stars, based on 1 article reviews
β3 polyclonal antibody - by Bioz Stars, 2026-11
90/100 stars
  Buy from Supplier
cosmc antibody  (biorbyt)
N/A
Rabbit polyclonal antibody to COSMC. Isotype Note: IgG Host Note: Rabbit Conjugation Note: Unconjugated Reactivity Note: Human, Mouse, Rat Application Note: ELISA, WB, IHC-P
   Buy from Supplier
cosmc antibody  (Abbexa)
N/A
COSMC Antibody is a Rabbit Polyclonal against COSMC
   Buy from Supplier

Image Search Results


Expression of aberrant O-glycans in pancreatic cancer. a Biosynthesis of Tn antigen, sTn antigen and Core1 and 3 structures. Tn antigen is composed of an O-glycosidic linked N -acetylgalactosamine (GalNAc) to the –OH group of serine/threonine (S/T). Tn antigen is either processed by core 1 T-synthase (C1GalT1) and its chaperone (COSMC), which transfers a galactose (Gal) to GalNAc-serine/threonine to form the T antigen also referred as core 1 structure or processed by transfer of a N -acetylglucosamine (GlcNAc) to form the core 3 structure. Tn antigen can also be modified by addition of a sialic acid (NeuAc). b Differential expression of Tn antigen in pancreatic carcinoma cell lines. Eight different PDAC cell lines were available for analysis. Western and Far-Western blot analysis of total cell lysates was performed using the Tn antigen specific antibody MA1-80055. Detection of HSC70 served as loading control. Jurkat cells were used as positive control for Tn antigen expression. c Expression of Tn antigen and aberrant O-glycans in COSMC knockdown cells. Western blot analysis showed a strong expression of aberrant O-glycans as well as Tn antigen in Panc-1 COSMC knockdown cells compared to control cells. Sialyl-Tn and Tn antibodies were used as well as lectins such as VVL ( Vicia villosa lectin) and WFL ( Wisteria floribunda lectin)

Journal: Molecular Cancer

Article Title: COSMC knockdown mediated aberrant O-glycosylation promotes oncogenic properties in pancreatic cancer

doi: 10.1186/s12943-015-0386-1

Figure Lengend Snippet: Expression of aberrant O-glycans in pancreatic cancer. a Biosynthesis of Tn antigen, sTn antigen and Core1 and 3 structures. Tn antigen is composed of an O-glycosidic linked N -acetylgalactosamine (GalNAc) to the –OH group of serine/threonine (S/T). Tn antigen is either processed by core 1 T-synthase (C1GalT1) and its chaperone (COSMC), which transfers a galactose (Gal) to GalNAc-serine/threonine to form the T antigen also referred as core 1 structure or processed by transfer of a N -acetylglucosamine (GlcNAc) to form the core 3 structure. Tn antigen can also be modified by addition of a sialic acid (NeuAc). b Differential expression of Tn antigen in pancreatic carcinoma cell lines. Eight different PDAC cell lines were available for analysis. Western and Far-Western blot analysis of total cell lysates was performed using the Tn antigen specific antibody MA1-80055. Detection of HSC70 served as loading control. Jurkat cells were used as positive control for Tn antigen expression. c Expression of Tn antigen and aberrant O-glycans in COSMC knockdown cells. Western blot analysis showed a strong expression of aberrant O-glycans as well as Tn antigen in Panc-1 COSMC knockdown cells compared to control cells. Sialyl-Tn and Tn antibodies were used as well as lectins such as VVL ( Vicia villosa lectin) and WFL ( Wisteria floribunda lectin)

Article Snippet: Tn antigen antibody (MA1-80055, clone BRIC111, Thermo Fischer), sTn antigen antibody (ABIN356328, clone STN218, antibodies online) and COSMC antibody (19254-1-AP, Proteintech) were diluted 1:250 in TBS-T. HSC70 (sc-7298), GAPDH (sc-32233,) Nucleolin (sc-8031), GRP78 (sc-1051), Alpha Enolase (sc-7455) and Annexin II (sc-9061) antibodies were purchased from Santa Cruz and diluted 1:1000.

Techniques: Expressing, Modification, Quantitative Proteomics, Western Blot, Far Western Blot, Control, Positive Control, Knockdown

MS identified Nucleolin, GRP-78, α-Enolase and Annexin A2 display O-GalNAc glycosylation. a left : Tn antigen is expressed on Nucleolin, GRP-78, α-Enolase and Annexin A2. Immunoprecipitation and western blot analysis were performed using VVL lectin for Tn antigen precipitation and Nucleolin, GRP-78, α-Enolase and Annexin A2 antibodies for detection of specific protein signals. a right : Protein expression levels of the identified proteins in total cell lysates of COSMC knockdown as well as control cells remained unchanged, except for Annexin 2, which showed a decrease in Panc-1 COSMC knockdown cells. b Vice versa, immunoprecipitation using specific antibodies and detection with VVL lectin was performed as well. c Immunocytochemistry of Panc-1 COSMC knockdown cells and control cells. Left : Aberrant O-glycans, including Tn antigen, were detected using VVL-FITC conjugated lectin ( green ). Middle left : Nucleolin was detected ( red ) using an anti-Nucleolin antibody (ab13541). Middle right: Nuclei were stained with DAPI ( blue ). Right : Overlay of Tn antigen and Nucleolin immunocytochemistry. d Nucleolin, GRP-78, Enolase and Annexin detection in the cell membrane protein fraction. Her2 antibody was used as marker for membrane fraction and GAPDH antibody was used as marker for cytoplasmic proteins

Journal: Molecular Cancer

Article Title: COSMC knockdown mediated aberrant O-glycosylation promotes oncogenic properties in pancreatic cancer

doi: 10.1186/s12943-015-0386-1

Figure Lengend Snippet: MS identified Nucleolin, GRP-78, α-Enolase and Annexin A2 display O-GalNAc glycosylation. a left : Tn antigen is expressed on Nucleolin, GRP-78, α-Enolase and Annexin A2. Immunoprecipitation and western blot analysis were performed using VVL lectin for Tn antigen precipitation and Nucleolin, GRP-78, α-Enolase and Annexin A2 antibodies for detection of specific protein signals. a right : Protein expression levels of the identified proteins in total cell lysates of COSMC knockdown as well as control cells remained unchanged, except for Annexin 2, which showed a decrease in Panc-1 COSMC knockdown cells. b Vice versa, immunoprecipitation using specific antibodies and detection with VVL lectin was performed as well. c Immunocytochemistry of Panc-1 COSMC knockdown cells and control cells. Left : Aberrant O-glycans, including Tn antigen, were detected using VVL-FITC conjugated lectin ( green ). Middle left : Nucleolin was detected ( red ) using an anti-Nucleolin antibody (ab13541). Middle right: Nuclei were stained with DAPI ( blue ). Right : Overlay of Tn antigen and Nucleolin immunocytochemistry. d Nucleolin, GRP-78, Enolase and Annexin detection in the cell membrane protein fraction. Her2 antibody was used as marker for membrane fraction and GAPDH antibody was used as marker for cytoplasmic proteins

Article Snippet: Tn antigen antibody (MA1-80055, clone BRIC111, Thermo Fischer), sTn antigen antibody (ABIN356328, clone STN218, antibodies online) and COSMC antibody (19254-1-AP, Proteintech) were diluted 1:250 in TBS-T. HSC70 (sc-7298), GAPDH (sc-32233,) Nucleolin (sc-8031), GRP78 (sc-1051), Alpha Enolase (sc-7455) and Annexin II (sc-9061) antibodies were purchased from Santa Cruz and diluted 1:1000.

Techniques: Glycoproteomics, Immunoprecipitation, Western Blot, Expressing, Knockdown, Control, Immunocytochemistry, Staining, Membrane, Marker